Do both halves of the 12AU7 light up? If the solder joint at A4 and A5 is dodgy and only half of the 12AU7 is lighting up, that will cause the problem you have.
If you're 100% certain both halves of the 12AU7 are illuminated, can you carefully measure the voltage at A3?
For most instances like this, we find that builders will zero in on the LED itself and resolder it until they destroy it, so resist the temptation to go nuts resoldering the LED itself.