With that voltage there, the 2A3 should be glowing, and you should see ~60V DC from pins 1 and 4 to ground. If you don't see the tube glowing, you will want to double check that both tubes don't glow in that socket, as well as that the socket is installed properly with the big pins facing the rear of the chassis (You mentioned that the initial voltage checks were OK, which would mean that the socket is where it needs to be).