Tube glow is controlled by pins 4 and 5 on the 6C45PI sockets and pins 4/5/9 on the 12AU7 socket.
Come to think of it, a loose connection at pin 4 or 5 of that 12AU7 socket could cause these issues.
Thanks. I've tried reheating those pins as well, but the issue did not resolve.
I also swapped the 6C45PI and re-inserted 12AU7.
I'm not sure the issue is a cold joint. I don't want to sound over confident about the joints but I have a bit of an OCD when it comes to soldering, I examined every single one meticulously and redid them if necessary. I use a Hakko workstation and its heat is pretty consistent.
Could it be a bad transistor? Based on other threads, the issue was usually a human error and not a faulty part, so this seems unlikely too.
Either way, I measured the voltages of the MJE5731A pins.
On the "good" C4S:
(E-C-B voltages)
A side (278V, 220V, 277V)
B side (217V, 144V, 217V)
On the faulty C4S:
A side (278V, 190V, 277V)
B side (142.5V, 142.5V, 141.8V)