So if you have that cathode load line up, you can see that with 325V of total B+ to work with, the 2A3-40 will settle with about 265V of B+, 50V of bias, and about 52mA of quiescent current. That 265 and 50 doesn't quite get to 325 because I am allotting some voltage drop for your output transformer.
In order to determine where you would land on the cathode resistance line, it was just a matter of finding the spot where the grid bias volts and the plate volts added up to about 325.