OK, this narrows the potential issues down to:
1. LED connection/functionality (shouldn't be a problem since the amp was working pre-Speedball).
2. A poor connection from the wire leaving the center lug of the volume control to that particular channel (worth double checking).
3. Issues within the C4S itself. I would quadruple check the ground connection to the board.
4. Issues with the wire leaving the "O" pad on the PCB, and heading to the 12AU7 (same as #2, just give it a good look over).
You can always do the chopstick test, find a wood or all plastic poking implement, then poke each transistor, LED, resistor, and wire leaving the offending PC board while the amp is running and the LED's are out. At some point, you will poke the right part and it will reveal itself as the problem.
-PB