The 10K potentiometer (R4) and the 4.99K (R3) resistor are on the outer end of the "A" side of the board. They are in series on the board, with the 431 chip in parallel. The ground end goes to -reg on the "A" end, which should go to signal ground. The hot end of this combination goes to kreg on the "A" side, which in turn goes to the tube socket pin B2, There is a problem with one of these connections.
Based on the 2.50 volt reading at kreg (A side) I suspect the 10K pot is OK but the 4.99K resistor is not connected at one end. Measure the resistance of the resistor itself to be sure the right part is being used, then check the solder connections.