I found the 5v rail on my computers USB bus to be incredibly noisy so i built a small box with an ADUM4160 isolator powered by a low noise TI TPS7A4700 regulator for my USB powered DACs. The difference it makes varies from one DAC to another but there is a small audible and measurable difference in my opinion.
The RMAA trace below shows a PupDAC (USB Powered) being fed direct from the PC, vs an isolator with a LM317 power supply (Beezar Doodlebug).
For the BH DAC the isolator has to go as its only USB 1.1, but i am going to measure it with the direct USB supply, vs my own USB power supply and see if it makes any difference at all. I suspect it wont from what John has said so far, he seems to have gone to great lengths to isolate the input side of things.