here is some info from the EML site about burning in tubes.
Yes they do! Good Burn-in will assure maximum lifetime and develop the final sound. These tubes are burned-in initially, to do the factory testing, after tube data becomes stabile. However emission of the cathode is not homogenous at this moment. Emission is build by many small islands, overlapping each other. This will not give the final sound yet. The burn-in process will take place in the first 50-100 hours under normal use conditions. This means it is best to switch off the amplifier after each use, and in the beginning not use the tubes longer than 4 hours at one time. Many short use periods have a better result than few long periods. The tubes need the „cold“ periods in between for best formatting of the filament. Some blue glow effect on the glass will disappear during burn-in, or may take longer to disappear eventually. What you observe, is a fluorescent effect, and it is normal with new tubes, even so proving cleanliness of the glass. For good burn in, use different loudness levels from the beginning, and increase the maximum loudness gradually. If tubes with very little use were switched off longer than 12...24 months, it may be necessary to repeat the burn in. So tubes that were not used for some years, may sound unpleasant, and simply need a new burn in.