I wonder whether the DC filament supply mod has lowered the noise floor of the amp, revealing "harshness" that was already there in the signal but was previously inaudible b/c it was buried in the noise floor. If so, this suggests a silver lining: the amp is now more resolving, and if Philip can hunt down the source of, or otherwise ameliorate, the harshness, he may have an even *better* sounding system. I'd start by looking at the source and pre-amp. Even the interconnect cables might make a difference (fairly easy to diy with different wire gauges, wire compositions, shielding vs. no-shielding). Or perhaps, lastly, a different set of 300Bs.
cheers, Derek